Polymerase chain reaction enrichment and normalization

Comparison of fine needle aspiration and direct skin smear in the diagnosis of acute cutaneous leishmaniasis: polymerase chain reaction as a reference method

Background: Accurate diagnosis of cutaneous leishmaniasis wouldavoid unnecessary treatment and scar formation. Direct smear isthe most common method for the diagnosis of this disease but itsnegative result could not rule out the infection; so, the need formore sensitive methods is obvious. We conducted this study tocompare the efficiency of direct skin smear with smears preparedby fine needle a...

Chainy: an universal tool for standardized relative quantification in real-time PCR

Summary Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utilit...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Letting all have a say: A novel method for microRNA RT- PCR normalization

MicroRNAs (miRNAs) are short non-coding RNA molecules. MicroRNAs regulate mRNA transcript levels and translation. miRNA expression is measured by microarray or real-time polymerase chain reaction (RT-PCR). The findings of RT-PCR data are limited by the normalization techniques. Some commonly used endogenous controls are differentially expressed in cancer, making them inappropriate internal cont...

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...

Error propagation in relative real-time reverse transcription polymerase chain reaction quantification models: the balance between accuracy and precision.

Real-time reverse transcription polymerase chain reaction (RT-PCR) has gained wide popularity as a sensitive and reliable technique for mRNA quantification. The development of new mathematical models for such quantifications has generally paid little attention to the aspect of error propagation. In this study we evaluate, both theoretically and experimentally, several recent models for relative...